Please check your mail server logs again. Possibly the above is triggered by auto testing.
Wihtout knowing why error 501 comes, it’s impossible to help.
We found the Problem.
The FQDN in Zammad (Settings => System) had a space in it. This isn´t allowed with Exchange. The next problem was that the Testmail was send to the Inbox, but we setup an own Folder, which was only checked.
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